Live vaccine heat-resistant protective agent as well as preparation method and application thereof

The invention relates to the technical field of biological medicines, and particularly discloses a heat-resistant protective agent for live vaccines as well as a preparation method and application of the heat-resistant protective agent. The live vaccine protective agent is prepared from synanthrin, gelatin, glycine, polyvinylpyrrolidone, bovine serum albumin, enzymolysis casein, D-sorbitol, water-soluble phospholipid, pine pollen and tocopherol. Wherein the mass ratio of the pine pollen to the tocopherol is (1-2): 1. The protective agent provided by the invention has the advantages of simple and effective components, safe and easily available raw materials and convenient preparation method, is used for preparing the mycoplasma bovis live vaccine, can effectively reduce the loss of the viable rate of each mycoplasma in the freeze drying process, prolongs the storage life, and induces the organism to generate long-lasting and efficient immune response. 本发明涉及生物医药技术领域，具体公开了一种活疫苗耐热保护剂及其制备方法与应用。本发明的活疫苗保护剂，包括：菊糖、明胶、