Homogeneous chemiluminescence determination kit for procalcitonin

The invention belongs to the technical field of in-vitro detection, and particularly relates to a homogeneous chemiluminescence determination kit for procalcitonin. The procalcitonin kit comprises a reagent R1 and a reagent R2 which are independent of each other, the reagent R1 comprises luminous pa...

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Bibliographische Detailangaben
Hauptverfasser: HUANG JINFA, YANG YIKUN, SHANG YIWEN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention belongs to the technical field of in-vitro detection, and particularly relates to a homogeneous chemiluminescence determination kit for procalcitonin. The procalcitonin kit comprises a reagent R1 and a reagent R2 which are independent of each other, the reagent R1 comprises luminous particles coated with a procalcitonin monoclonal antibody, a surfactant A and a stabilizer, and the reagent R2 comprises a biotin-labeled procalcitonin monoclonal antibody and a surfactant B, the surfactant A comprises a mixture of Tween 80 and PVP K30 in a mass ratio of (1-2): 1, and the surfactant B comprises a mixture of triton x-100 and polyoxyethylene alkyl ether EMULGEN 709 in a mass ratio of 1: (2-4). After the surfactant A and the surfactant B in a specific mass ratio are added, a relatively good component enhancing effect can be achieved; by reasonably controlling the content of each substance in the R1 reagent and the R2 reagent, the accuracy, precision, stability and specificity of the procalcitonin determ