Method for exploring peripheral proteins of leading chain and trailing chain based on APEX2 proximity labeling technology

The invention discloses a method for exploring peripheral proteins of a leading chain and a following chain based on an APEX2 proximity marker technology, which comprises the following steps of: constructing a fusion protein carrier containing an EFI alpha promoter sequence, APEX2 and V5 by selecting a key protein related to DNA (Deoxyribonucleic Acid) copying the leading chain and the following chain as a target, and transforming the fusion protein carrier into a host cell line to form a fusion cell line; the method comprises the following steps: performing biotin labeling on adjacent proteins through biotin-phenol and hydrogen peroxide treatment, then extracting and purifying biotinylated proteins, performing quality verification, and finally performing mass spectrum and chromatography on-machine analysis. The improved APEX2 proximity marking technology is used, the defects that other proximity technologies are large in marking radius and long in experiment time are overcome, and efficiency is improved. The