TaqMan fluorescent quantitative PCR (Polymerase Chain Reaction) primer group and method for detecting pangolin parainfluenza virus type 5
The invention discloses a TaqMan fluorescent quantitative PCR (Polymerase Chain Reaction) primer group and a method for detecting pangolin parainfluenza virus type 5. The kit disclosed by the invention comprises a pair of specific primers, a TaqMan probe and the like aiming at the NP protein gene of...
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| creator | ZHU LINGJUN LI JUN HOU FANGHUI ZHANG LINA WANG KAI WANG HE HUANG WANHE |
| description | The invention discloses a TaqMan fluorescent quantitative PCR (Polymerase Chain Reaction) primer group and a method for detecting pangolin parainfluenza virus type 5. The kit disclosed by the invention comprises a pair of specific primers, a TaqMan probe and the like aiming at the NP protein gene of the pangolin parainfluenza virus type 5; the kit is good in amplification curve and high in specificity, and has no cross reaction with other viruses capable of infecting the pangolin except the pangolin parainfluenza virus type 5; when a positive standard substance diluted by 10 times of gradient is detected, the sensitivity is high, and the lowest detection limit is 2.35 * 10 < 1 > copies/mu L; the TaqMan fluorescent quantitative PCR detection method adopted by the invention has the advantages of high sensitivity, strong specificity, short time consumption and the like.
本发明公开了一种检测穿山甲副流感病毒5型的TaqMan荧光定量PCR引物组和方法。本发明的试剂盒包括针对穿山甲副流感病毒5型的NP蛋白基因的一对特异性引物和TaqMan探针等；该试剂盒扩增曲线良好，特异性强，与除穿山甲副流感病毒5型之外的其他可感染穿山甲的病毒没有交叉反应；检测10倍梯度 |
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本发明公开了一种检测穿山甲副流感病毒5型的TaqMan荧光定量PCR引物组和方法。本发明的试剂盒包括针对穿山甲副流感病毒5型的NP蛋白基因的一对特异性引物和TaqMan探针等；该试剂盒扩增曲线良好，特异性强，与除穿山甲副流感病毒5型之外的其他可感染穿山甲的病毒没有交叉反应；检测10倍梯度</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; COMPOSITIONS THEREOF ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; CULTURE MEDIA ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2024</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20240628&DB=EPODOC&CC=CN&NR=118256659A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25562,76317</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20240628&DB=EPODOC&CC=CN&NR=118256659A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>ZHU LINGJUN</creatorcontrib><creatorcontrib>LI JUN</creatorcontrib><creatorcontrib>HOU FANGHUI</creatorcontrib><creatorcontrib>ZHANG LINA</creatorcontrib><creatorcontrib>WANG KAI</creatorcontrib><creatorcontrib>WANG HE</creatorcontrib><creatorcontrib>HUANG WANHE</creatorcontrib><title>TaqMan fluorescent quantitative PCR (Polymerase Chain Reaction) primer group and method for detecting pangolin parainfluenza virus type 5</title><description>The invention discloses a TaqMan fluorescent quantitative PCR (Polymerase Chain Reaction) primer group and a method for detecting pangolin parainfluenza virus type 5. The kit disclosed by the invention comprises a pair of specific primers, a TaqMan probe and the like aiming at the NP protein gene of the pangolin parainfluenza virus type 5; the kit is good in amplification curve and high in specificity, and has no cross reaction with other viruses capable of infecting the pangolin except the pangolin parainfluenza virus type 5; when a positive standard substance diluted by 10 times of gradient is detected, the sensitivity is high, and the lowest detection limit is 2.35 * 10 < 1 > copies/mu L; the TaqMan fluorescent quantitative PCR detection method adopted by the invention has the advantages of high sensitivity, strong specificity, short time consumption and the like.
本发明公开了一种检测穿山甲副流感病毒5型的TaqMan荧光定量PCR引物组和方法。本发明的试剂盒包括针对穿山甲副流感病毒5型的NP蛋白基因的一对特异性引物和TaqMan探针等；该试剂盒扩增曲线良好，特异性强，与除穿山甲副流感病毒5型之外的其他可感染穿山甲的病毒没有交叉反应；检测10倍梯度</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>COMPOSITIONS THEREOF</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2024</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNjjEOgkAQRWksjHqHsdPCAg1ES0M0NhpC6MkEBtgEZpfdgQRv4K3dwgNY_eK_l_-XwSfH4YkMdTdqS64kFhhGZFGCoiaCNMlgl-pu7smiI0haVAwZYSlK8x6MVb6BxurRAHIFPUmrK6i1hYqEPMYNGORGd140aL3v14jfCJOyowOZDUG0DhY1do42v1wF2_stTx4HMrogZ9B_IymSVxiej1EcR5fr6R_mCwiZTIY</recordid><startdate>20240628</startdate><enddate>20240628</enddate><creator>ZHU LINGJUN</creator><creator>LI JUN</creator><creator>HOU FANGHUI</creator><creator>ZHANG LINA</creator><creator>WANG KAI</creator><creator>WANG HE</creator><creator>HUANG WANHE</creator><scope>EVB</scope></search><sort><creationdate>20240628</creationdate><title>TaqMan fluorescent quantitative PCR (Polymerase Chain Reaction) primer group and method for detecting pangolin parainfluenza virus type 5</title><author>ZHU LINGJUN ; LI JUN ; HOU FANGHUI ; ZHANG LINA ; WANG KAI ; WANG HE ; HUANG WANHE</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN118256659A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2024</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>COMPOSITIONS THEREOF</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>ZHU LINGJUN</creatorcontrib><creatorcontrib>LI JUN</creatorcontrib><creatorcontrib>HOU FANGHUI</creatorcontrib><creatorcontrib>ZHANG LINA</creatorcontrib><creatorcontrib>WANG KAI</creatorcontrib><creatorcontrib>WANG HE</creatorcontrib><creatorcontrib>HUANG WANHE</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>ZHU LINGJUN</au><au>LI JUN</au><au>HOU FANGHUI</au><au>ZHANG LINA</au><au>WANG KAI</au><au>WANG HE</au><au>HUANG WANHE</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>TaqMan fluorescent quantitative PCR (Polymerase Chain Reaction) primer group and method for detecting pangolin parainfluenza virus type 5</title><date>2024-06-28</date><risdate>2024</risdate><abstract>The invention discloses a TaqMan fluorescent quantitative PCR (Polymerase Chain Reaction) primer group and a method for detecting pangolin parainfluenza virus type 5. The kit disclosed by the invention comprises a pair of specific primers, a TaqMan probe and the like aiming at the NP protein gene of the pangolin parainfluenza virus type 5; the kit is good in amplification curve and high in specificity, and has no cross reaction with other viruses capable of infecting the pangolin except the pangolin parainfluenza virus type 5; when a positive standard substance diluted by 10 times of gradient is detected, the sensitivity is high, and the lowest detection limit is 2.35 * 10 < 1 > copies/mu L; the TaqMan fluorescent quantitative PCR detection method adopted by the invention has the advantages of high sensitivity, strong specificity, short time consumption and the like.
本发明公开了一种检测穿山甲副流感病毒5型的TaqMan荧光定量PCR引物组和方法。本发明的试剂盒包括针对穿山甲副流感病毒5型的NP蛋白基因的一对特异性引物和TaqMan探针等；该试剂盒扩增曲线良好，特异性强，与除穿山甲副流感病毒5型之外的其他可感染穿山甲的病毒没有交叉反应；检测10倍梯度</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | TaqMan fluorescent quantitative PCR (Polymerase Chain Reaction) primer group and method for detecting pangolin parainfluenza virus type 5 |
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