TaqMan fluorescent quantitative PCR (Polymerase Chain Reaction) primer group and method for detecting pangolin parainfluenza virus type 5

The invention discloses a TaqMan fluorescent quantitative PCR (Polymerase Chain Reaction) primer group and a method for detecting pangolin parainfluenza virus type 5. The kit disclosed by the invention comprises a pair of specific primers, a TaqMan probe and the like aiming at the NP protein gene of the pangolin parainfluenza virus type 5; the kit is good in amplification curve and high in specificity, and has no cross reaction with other viruses capable of infecting the pangolin except the pangolin parainfluenza virus type 5; when a positive standard substance diluted by 10 times of gradient is detected, the sensitivity is high, and the lowest detection limit is 2.35 * 10 < 1 > copies/mu L; the TaqMan fluorescent quantitative PCR detection method adopted by the invention has the advantages of high sensitivity, strong specificity, short time consumption and the like. 本发明公开了一种检测穿山甲副流感病毒5型的TaqMan荧光定量PCR引物组和方法。本发明的试剂盒包括针对穿山甲副流感病毒5型的NP蛋白基因的一对特异性引物和TaqMan探针等；该试剂盒扩增曲线良好，特异性强，与除穿山甲副流感病毒5型之外的其他可感染穿山甲的病毒没有交叉反应；检测10倍梯度