DsRNA with prevention and treatment effects on botrytis cinerea and fusarium oxysporum as well as preparation method and application of dsRNA

The invention discloses dsRNA (double-stranded ribonucleic acid) with prevention and treatment effects on botrytis cinerea and fusarium oxysporum as well as a preparation method and application of the dsRNA. The key pathogenic genes of the botrytis cinerea and the fusarium oxysporum which are subjec...

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Bibliographische Detailangaben
Hauptverfasser:	ZHAO HONGWEI, SHI XUECHAO, XU LE, DU RUONAN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PEST REPELLANTS OR ATTRACTANTS PLANT GROWTH REGULATORS PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRAPPING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses dsRNA (double-stranded ribonucleic acid) with prevention and treatment effects on botrytis cinerea and fusarium oxysporum as well as a preparation method and application of the dsRNA. The key pathogenic genes of the botrytis cinerea and the fusarium oxysporum which are subjected to precise targeted inhibition are a botrytis cinerea NADPH oxidase BcNoxb gene and a fusarium oxysporum NADPH oxidase FoNox1 gene respectively. The dsRNA is the dsRNA for precisely inhibiting the BcNoxb gene and the FoNox1 gene in a targeted manner. The precise targeted inhibition on the key virulence genes of botrytis cinerea and fusarium oxysporum provided by the invention is realized by inducing target gene expression silencing through exogenous application of dsRNA. The dsRNA accurately induces silencing of key pathogenic genes, so that the pathogenicity of botrytis cinerea and fusarium oxysporum is reduced or even lost, and the aim of protecting crops is achieved. The invention provides a new prevention a