Hybridoma cell preparation method based on electrofusion technology and application thereof

The invention relates to a preparation method and application of hybridoma cells based on an electrofusion technology, the preparation method of the hybridoma cells based on the electrofusion technology comprises the following steps: taking splenocytes and myeloma cells of an antigen-immunized mouse for electrofusion, screening to obtain the hybridoma cells, and the antigen is serum amyloid protein A. The invention also relates to a preparation method of the hybridoma cells based on the electrofusion technology. The hybridoma cell capable of generating the serum amyloid protein A antibody is obtained in an electrofusion mode, the fusion efficiency of the hybridoma cell is improved, and the problem that the yield of the antibody is reduced due to the fact that the fusion efficiency of the hybridoma cell is low is solved. 本发明涉及一种基于电融合技术的杂交瘤细胞制备方法及其应用，所述基于电融合技术的杂交瘤细胞制备方法包括：取抗原免疫后小鼠的脾细胞和骨髓瘤细胞进行电融合，筛选后得到所述杂交瘤细胞，所述抗原为血清淀粉样蛋白A。本发明通过电融合方式获得能够产生血清淀粉样蛋白A抗体的杂交瘤细胞，提高了杂交瘤细胞的融合效率，解决了因杂交瘤细胞融合效率低下导致的抗体产量降低的问题。