Monoclonal antibody aiming at human cone-like protein 1, antibody pair, kit and application thereof

The invention belongs to the technical field of antibody preparation, and particularly relates to a monoclonal antibody aiming at human cone-like protein 1, an antibody pair, a kit and application thereof. The monoclonal antibody is a first antibody or a second antibody, amino acid sequences of CDR1-3 on a light chain of the first antibody are respectively shown as SEQ ID NO.3-5, and amino acid sequences of CDR1-3 on a heavy chain of the first antibody are respectively shown as SEQ ID NO.8-10; amino acid sequences of CDR1-3 on a light chain of the second antibody are respectively shown as SEQ ID NO.13-15, and amino acid sequences of CDR1-3 on a heavy chain of the second antibody are respectively shown as SEQ ID NO.18-20. The antibody provided by the invention has high binding activity and affinity to human VILIP-1 protein, can form a double-antibody sandwich enzyme-linked immunosorbent assay system, and realizes specific, sensitive, reliable and accurate detection of very trace VILIP-1 in a sample. 本发明属于抗体制备技