Method for constructing strand-specific transcriptome library

The invention relates to the technical field of transcriptome library construction, in particular to a method for constructing a chain-specific transcriptome library. The method comprises the following steps: (1) enriching mRNA in a sample to be detected; (2) carrying out fragmentation treatment on the enriched mRNA; (3) taking the fragmented mRNA as a template, continuously synthesizing a first-chain cDNA and a second-chain cDNA in a synthetic reaction solution to generate double-chain cDNA, and continuing to repair the tail end of the double-chain cDNA and add A in the synthetic reaction solution; (4) connecting the double-chain cDNA added with A with a linker to obtain a linker connection product; and (5) performing library amplification on the linker connection product. According to the method, the synthesis of the first-chain cDNA and the second-chain cDNA, the end repair of the double-chain cDNA fragment and the end A addition reaction of the double-chain cDNA fragment are completed in one step in the s