Gossypium hirsutum genome editing expression vector as well as construction method and application thereof

The invention relates to the technical field of gene engineering, in particular to an upland cotton genome editing expression vector and a construction method and application thereof, the expression vector is pGhRBE3-pGh alpha GloA-GhPGF, and the nucleotide sequence of pGh alpha GloA in the expression vector pGhRBE3-pGh alpha GloA-GhPGF is shown as SEQ ID NO.1. The invention also relates to a construction method and application of the upland cotton genome editing expression vector. The invention aims to construct a pGhRBE3-pGh alpha GloA-GhPGF expression vector by adopting a cotton endogenous pGh alpha GloA promoter to drive crRNAs of a GhPGF gene, so that a cotton CRISPR/Cas12a gene editing system is optimized, the editing efficiency of the gene editing system for a single crRNA is up to 100%, and the efficiency of the gene editing system for simultaneously editing two crRNAs can reach 66.7%, thereby providing a new scheme for cotton genome editing. 本发明涉及基因工程技术领域，尤其涉及一种陆地棉基因组编辑表达载体及其构建方法与应用，所述表达载体为pGhRBE3-pG