Single-base gene editor pBE-C2A-T3 as well as preparation method and application thereof

The invention discloses a single-base gene editor pBE-C2A-T3 as well as a preparation method and application of the single-base gene editor pBE-C2A-T3. The single-base gene editor pBE-C2A-T3 comprises a fusion protein and a gRNA scaffold, wherein the fusion protein is a fusion protein; the nucleotide sequence of the coding gene of the fusion protein is as shown in SEQ ID NO. 1; and the nucleotide sequence of the gRNA scaffold is as shown in SEQ ID NO. 3. The single-base editing tool disclosed by the invention contains a plurality of nucleotide sequences for coding fusion protein, and the fusion protein mainly comprises Cas9Nickase, Linker, alkyl adenine DNA (Deoxyribose Nucleic Acid) glycosylase, cytidine deaminase and adenosine deaminase. According to the single-base editing tool pBE-C2A-T3, conversion of specific bases C-to-T and/or A-to-T can be achieved, the function of single base mutation can be reserved, conversion of two bases can be achieved at the same time, and the editing efficiency is higher. 本发明