Culture method for simulating microgravity to induce primary cancer cell reprogramming and application thereof

The invention relates to a culture method for simulating microgravity to induce primary cancer cell reprogramming and application of the culture method, and belongs to the technical field of biology. According to the novel primary cancer cell culture technology provided by the invention, a 10-3g microgravity environment is simulated by using a multi-directional G force generator, reprogramming of patient-derived carcinoma/para-carcinoma tissue cells is induced, dryness of a primary cell group is maintained, and rapid, long-term and high-fidelity culture of the patient-derived carcinoma/para-carcinoma tissue cells is realized. According to the SMGIR culture method for simulating microgravity induced reprogramming researched and developed by the project, tissue blocks with the size of about 1mm < 3 > are obtained from an operation for digestion culture until 106 cells are obtained to support an in-vitro drug screening experiment, only 7 days are needed, and the cultured cells can still be repeatedly cultured by