Engineered Escherichia coli using cane sugar or molasses and method for producing branched chain amino acid by fermentation of engineered Escherichia coli

The invention relates to escherichia coli engineering bacteria utilizing cane sugar or molasses and a method for producing branched chain amino acid through fermentation of the escherichia coli engineering bacteria, a genetic engineering strain is obtained by adopting a genetic engineering method, a...

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Bibliographische Detailangaben
Hauptverfasser:	ZHU XIANGCHENG, DAI ANDI, WANG JINGYU, ZHANG KECHUN, SUN CUNMIN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE FODDER FOODS OR FOODSTUFFS FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J HUMAN NECESSITIES METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT THEIR TREATMENT, NOT COVERED BY OTHER CLASSES VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to escherichia coli engineering bacteria utilizing cane sugar or molasses and a method for producing branched chain amino acid through fermentation of the escherichia coli engineering bacteria, a genetic engineering strain is obtained by adopting a genetic engineering method, and the genetic engineering strain substantially expresses a cane sugar metabolism gene. Comprising sucrose permease, sucrose hydrolase, PEP-sucrose phosphotransferase system enzyme II, sucrose phosphate hydrolase and fructokinase. Or the genetic engineering strain is an E.coli W (ATCC 9637) strain in which the cscR gene is substantially inactivated. The genetic engineering strain is subjected to fermentation culture by taking cane sugar or molasses as a carbon source, and branched chain amino acids (L-valine, L-isoleucine and L-leucine) are separated from a culture after the culture is finished. The invention also discloses a genetic engineering strain for producing branched chain amino acids (L-valine, L-isoleucin