Anti-human HMGA2 protein Ser105 site phosphorylated antibody and application thereof

The invention discloses an anti-human HMGA2 protein Ser105 site phosphorylated antibody and application thereof. The phosphorylated antibody is prepared by the following steps: determining an anti-human HMGA2Ser105 protein phosphorylated site; the method comprises the following steps: designing a to...

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Bibliographische Detailangaben
Hauptverfasser:	CHEN XIAOJING, LYU WEIGUO, GU LINGKAI, SHEN ZHANGJIN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PHYSICS PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses an anti-human HMGA2 protein Ser105 site phosphorylated antibody and application thereof. The phosphorylated antibody is prepared by the following steps: determining an anti-human HMGA2Ser105 protein phosphorylated site; the method comprises the following steps: designing a to-be-synthesized phosphorylated polypeptide sequence and a to-be-synthesized non-phosphorylated polypeptide sequence according to an HMGA2 target sequence and an anti-human HMGA2Ser105 protein phosphorylation site; synthesizing a corresponding polypeptide according to the phosphorylated polypeptide sequence and the non-phosphorylated polypeptide sequence, and coupling the polypeptide with a carrier protein to obtain an antigen synthetic peptide as an antigen; immunizing an animal with the antigen synthetic peptide and collecting antiserum; and purifying and identifying the antiserum to obtain the anti-human HMGA2 protein Ser105 site phosphorylated antibody. According to the invention, the expression difference of no