Near-infrared two-region fluorescence contrast agent and application thereof

The invention relates to a near-infrared two-region fluorescence contrast agent and application thereof, and belongs to the technical field of fluorescence imaging. The invention provides a compound PF8CN, the compound PF8CN has the characteristic of aggregation-induced fluorescence efficiency enhancement, the fluorescence quantum yield is enhanced by inhibiting the formation of H-aggregate in the self-assembly process, the brightness of the near-infrared two-region fluorescence contrast agent is further improved, and the compound PF8CN can be used for preparing the near-infrared two-region fluorescence contrast agent. PF8CN has the characteristic of inhibiting formation of H-aggregates in the self-assembly process, the prepared nano particles PF8CN NPs have extremely high NIR-II brightness, long-time and real-time NIR-II brain and hind limb blood vessel fluorescence imaging can be carried out, and the imaging effect is far better than that of indocyanine green. In addition, PF8CN NPs can also be used as a me