Compositions, methods and systems for non-invasive prenatal testing

The present disclosure provides devices, methods, and systems for performing a non-invasive prenatal test (NTPT) digital assay when producing at least a large number of counts/chromosomes for a set of chromosomes present in a sample, wherein performing the NIPT digital assay may include: distributing nucleic acids of the sample and material for an amplification reaction over a plurality of partitions; amplifying the nucleic acid with the material within the plurality of partitions; and upon detection of signals from the plurality of partitions, generating a count/chromosome. The present invention enables the processing of samples with unprecedented zoning, reaction, readout and analytical performance for NIPT digital analysis and/or other digital analysis involving other loci of interest. 本公开提供了用于在为样品中存在的染色体集产生至少大量的计数/染色体时进行无创产前测试(NIPT)数字测定的装置、方法和系统，其中进行所述NIPT数字测定可以包括：在多个分区上分布所述样品的核酸和用于扩增反应的材料；在所述多个分区内用所述材料扩增所述核酸；以及在检测到来自所述多个分区的信号时，产生计数/染色体。本发明能够以前所未有的分区、反应、读出和分析性能处理样品用于NIPT数字分析和/或涉及其他目的基因座的其他数字分析。