Quantitative detection method for hydroxylamine hydrochloride in macrolide compound

The invention provides a quantitative detection method for hydroxylamine hydrochloride in macrolide compounds, and belongs to the technical field of chromatographic separation and analysis. The method comprises the following steps: loading a macrolide compound into a centrifuge tube, sequentially adding dichloromethane and water, uniformly mixing, centrifuging, taking a water layer, adding an acetic acid solution and a benzaldehyde solution, uniformly mixing, standing at room temperature to obtain a sample, and feeding the sample into a liquid chromatograph under the conditions that the sample injection amount is 50 microliters, the flow velocity is 0.9-1.1 ml/min, the column temperature is 35-45 DEG C and the detection wavelength is 250-260 nm; the mobile phase A is a formic acid aqueous solution, and the mobile phase B is a formic acid acetonitrile solution. By adopting the method disclosed by the invention, the defect that hydroxylamine hydrochloride has no response or is low in response in a common instru