Directional improvement method for complex rice characters

The invention provides a directional improvement method for complex rice characters, which is based on the combination of a multi-gene editing technology and traditional hybridization breeding, and provides a gene editing material creation process comprising a one-step PCR (Polymerase Chain Reaction...

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Bibliographische Detailangaben
Hauptverfasser: XIE HUAAN, WU FANGXI, LUO XI, XIAO YANJIA, XIE HONGGUANG, ZHENG YANMEI, ZHANG JIANFU, CAI QIUHUA, WEI YIDONG
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention provides a directional improvement method for complex rice characters, which is based on the combination of a multi-gene editing technology and traditional hybridization breeding, and provides a gene editing material creation process comprising a one-step PCR (Polymerase Chain Reaction) method multi-gene assembling system, a progeny rapid fluorescence screening method, high-throughput multi-gene typing and the like. Strain materials with genetic stability and excellent characters can be obtained only in short two years. And a feasible case is provided for subsequent multi-gene pyramiding breeding. According to the method, a strategy for directional improvement of complex agronomic traits is established, a rapid and practical process from sgRNA assembly and non-transgenosis screening to promising germplasm resource creation through combination of MGE and breeding is provided, and directional improvement of the growth period of main excellent cultivated varieties can be achieved under the conditio