Monoclonal antibody IG2 for resisting Penton protein of fowl adenovirus 4 and application of monoclonal antibody IG2
The invention discloses a monoclonal antibody IG2 for resisting Penton protein of fowl adenovirus type 4 and application of the monoclonal antibody IG2, the monoclonal antibody IG2 disclosed by the invention is secreted by a fowl adenovirus type IV hybridoma cell strain IG2, and the preservation num...
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Zusammenfassung: | The invention discloses a monoclonal antibody IG2 for resisting Penton protein of fowl adenovirus type 4 and application of the monoclonal antibody IG2, the monoclonal antibody IG2 disclosed by the invention is secreted by a fowl adenovirus type IV hybridoma cell strain IG2, and the preservation number of the fowl adenovirus type IV hybridoma cell strain IG2 in the China Center for Type Culture Collection is CCTCC NO: C2023247; an inactivated virus is used as an immunogen, a recombinant Penton protein is used as a detection antigen, a monoclonal antibody strain aiming at the Penton protein is finally screened out through three times of mouse immunization and two times of subcloning, subtype identification shows that a heavy chain is IgG2a type, a light chain is Kappa type, Western blot identification and FAdV-4 IPMA identification are carried out on the monoclonal antibody strain and the Penton protein, the monoclonal antibody strain can react, the ascites titer reaches 1: 7000, the stability is good, and the |
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