Application of fermentation metabolite of huperzia serrata endophytic fungus Alternania alternania FL7 in resisting acetylcholin esterase activity

The invention provides an application of a fermentation metabolite of a huperzia serrata endophytic fungus Alternania alternania FL7 in resisting the activity of acetylcholin esterase, and belongs to the field of microbiology and chemistry. The method comprises the following steps: dissolving an extract obtained by extracting a rice fermentation product of endophytic fungi FL7 with methanol in ultrapure water, and extracting for three times with petroleum ether and ethyl acetate respectively; eluting the ethyl acetate extract with petroleum ether and ethyl acetate in different volume ratios to obtain fractions Fr.1 to Fr.4; eluting the fraction Fr.1 with petroleum ether and ethyl acetate in different volume ratios to obtain fractions Fr.1. 1 to Fr.1. 4; the fraction Fr.1. 3 is eluted with cyclohexane and ethyl acetate according to the volume ratio of 10: 1 to obtain Fr1.3. 1, and finally the Fr1.3. 1 is purified with chloroform and methanol according to the volume ratio of 1: 1 to obtain a white needle-shaped