Quantitative analysis method of protein and polypeptide applied to xylose production

Quantitative analysis method of protein and polypeptide applied to xylose production

The invention discloses a quantitative analysis method of protein and polypeptide applied to xylose production, which belongs to the field of material detection of xylose production, and comprises the following steps: collecting a sample in time, adjusting the pH value to be alkaline, preparing a st...

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Hauptverfasser: LI ZHE, LIU PENG, LUO XITAO, ZHANG ZUOJIANG, LYU XINGHUA, MENG LINGKUN, ZHANG WEIHONG
Format: Patent
Sprache:chi ; eng
Schlagworte:
CALCULATING
COMPUTING
COUNTING
ELECTRIC DIGITAL DATA PROCESSING
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
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creator LI ZHE
LIU PENG
LUO XITAO
ZHANG ZUOJIANG
LYU XINGHUA
MENG LINGKUN
ZHANG WEIHONG
description The invention discloses a quantitative analysis method of protein and polypeptide applied to xylose production, which belongs to the field of material detection of xylose production, and comprises the following steps: collecting a sample in time, adjusting the pH value to be alkaline, preparing a standard solution by using plant protein, adding the standard solution into a coomassie brilliant blue G-250 solution, fully and uniformly mixing, standing for 3-7 minutes, and measuring the content of the protein and the polypeptide in the sample; a standard solution containing 0 microgram of standard protein is used as a blank control within 25-35 minutes, absorbance is measured at 595 nm, a standard curve is drawn according to the standard protein concentration in the standard solution and the corresponding absorbance, the correlation coefficient of a linear regression equation is not lower than 0.99, a blank solution of a coomassie brilliant blue G-250 solution is prepared, and the coomassie brilliant blue G-250
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subjects CALCULATING
COMPUTING
COUNTING
ELECTRIC DIGITAL DATA PROCESSING
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
title Quantitative analysis method of protein and polypeptide applied to xylose production
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