Quantitative analysis method of protein and polypeptide applied to xylose production

The invention discloses a quantitative analysis method of protein and polypeptide applied to xylose production, which belongs to the field of material detection of xylose production, and comprises the following steps: collecting a sample in time, adjusting the pH value to be alkaline, preparing a standard solution by using plant protein, adding the standard solution into a coomassie brilliant blue G-250 solution, fully and uniformly mixing, standing for 3-7 minutes, and measuring the content of the protein and the polypeptide in the sample; a standard solution containing 0 microgram of standard protein is used as a blank control within 25-35 minutes, absorbance is measured at 595 nm, a standard curve is drawn according to the standard protein concentration in the standard solution and the corresponding absorbance, the correlation coefficient of a linear regression equation is not lower than 0.99, a blank solution of a coomassie brilliant blue G-250 solution is prepared, and the coomassie brilliant blue G-250