Treatment method and application of red blood cell-containing paraffin tissue section for fluorescence in situ hybridization

The invention relates to a treatment method and application of a red blood cell-containing paraffin tissue section for fluorescence in situ hybridization, and belongs to the technical field of pathological tissue detection. According to the treatment method, after repairing treatment is completed, t...

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Hauptverfasser: GUO QIN, CHU ZHENNI, LI SANHUA, GUO SHUGUANG, LI DANYANG, XIE XIA, LI GUIXI, QI HUA, HUO QINGYUAN, ZHANG SEN, HE MENGXIAO
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention relates to a treatment method and application of a red blood cell-containing paraffin tissue section for fluorescence in situ hybridization, and belongs to the technical field of pathological tissue detection. According to the treatment method, after repairing treatment is completed, the paraffin tissue section before enzyme treatment digestion treatment is treated with an ascorbic acid solution, so that background fluorescence caused by red blood cells is weakened or eliminated. According to the treatment method for the hyperemia tissue section, the paraffin tissue section subjected to pretreatment liquid repair treatment is treated with the ascorbic acid solution, so that heme is reduced and degraded, and the hemoglobin structure is changed, and therefore digestion of protease on hemoglobin is facilitated; therefore, background fluorescence caused by red blood cells is weakened or eliminated, the hybridization effect of fluorescence in situ hybridization is ensured, and observation of fluoresc