Method for quantitatively detecting gene content of chimeric antigen receptor and application of method

The primer probe and the kit designed in the invention can accurately detect a CAR gene containing a coded CD19 antigen recognition peptide and a coded targeted CD19, are high in detection accuracy and sensitivity, have relatively high reference value for quality control release detection of a CAR-T cell preparation and in-vivo pharmacokinetic detection, and have good application prospects. The CAR-T quality control time can be shortened, distribution and continuation of the medicine can be accurately judged in vivo, guidance data is provided for effectiveness and safety of the medicine, and the method is suitable for application and popularization. 本发明中设计的引物探针以及试剂盒可以准确检测包含编码CD19抗原识别肽以及编码靶向CD19的CAR基因，且检测准确灵敏度高，对CAR-T细胞制剂的质量控制放行检测、体内的药代动力学检测具有较高的参考价值，可以缩短CAR-T质控时间以及体内准确判断药物的分布以及存续，进而为药物的有效性和安全性提供指导数据，适于推广应用。