Calculation method for movement speed of caenorhabditis elegans

The invention discloses a method for calculating the movement speed of caenorhabditis elegans. The method comprises the following steps: a, calibrating; b, image acquisition: shooting the whole culture dish in which no more than 30 caenorhabditis elegans are cultured to obtain a caenorhabditis elega...

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Bibliographische Detailangaben
Hauptverfasser: ZHANG JIACHEN, ZHANG ZHENG, JIAN YANYING, LI WEIYU, LIN QIZHAO, ZHAO XUAN, CHENG FANGYU, CHEN SUXIA, ZHU HUIQUAN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a method for calculating the movement speed of caenorhabditis elegans. The method comprises the following steps: a, calibrating; b, image acquisition: shooting the whole culture dish in which no more than 30 caenorhabditis elegans are cultured to obtain a caenorhabditis elegans group movement video; c, dynamic tracking: adopting a YOLO + DeepSort algorithm to realize dynamic tracking of the caenorhabditis elegans; d, data extraction: sorting to obtain information of the identified nematodes, wherein the information comprises nematode IDs; a timing sequence; e, speed calculation: firstly, according to nematode ID and time sequence sorting, deleting data of which the nematode ID number is greater than the number of caenorhabditis elegans; secondly, the displacement amount # imgabs0 # and the movement duration # imgabs1 # of each nematode are calculated respectively; calculating the displacement and the movement time of all the nematodes with the IDs, and obtaining the average movement sp