Method for identifying buckwheat plants
The invention discloses a method for identifying buckwheat plants. The method comprises the following steps: (1) extracting total DNA (Deoxyribose Nucleic Acid) from a to-be-identified sample; (2) taking the total DNA as a template, and carrying out PCR (Polymerase Chain Reaction) amplification by u...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention discloses a method for identifying buckwheat plants. The method comprises the following steps: (1) extracting total DNA (Deoxyribose Nucleic Acid) from a to-be-identified sample; (2) taking the total DNA as a template, and carrying out PCR (Polymerase Chain Reaction) amplification by using primers as shown in SEQ ID NO.1 and SEQ ID NO.2; (3) performing proofreading and splicing on the sequencing peak map of the amplification product, removing low-quality sequences and primer regions, performing multiple sequence comparison, removing redundant sequences at two ends, and applying the processed sequences to sequence analysis; and calculating the K-2-P distance of the sequences, analyzing and comparing the interspecific genetic distance of the sequences, and constructing a phylogenetic tree, so that buckwheat species are distinguished by clustering MATK sequences of different buckwheat species. The matK gene sequence is used for species identification, so that experimental data has enough reliabilit |
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