Method for preparing whole genome R-loop sequencing library

The invention relates to the technical field of biology, in particular to a method for preparing a whole genome R-loop sequencing library and a whole genome R-loop detection method. According to the invention, a library building scheme which can be widely compatible with various materials and can accurately and stably detect the R-loop site is established. An efficient genome extraction scheme is developed, double-stranded and single-stranded RNA and a large amount of impurities can be removed, meanwhile, R-loop is reserved, and the yield is extremely high; according to the library building method, a proper fragmentation scheme is selected, and sequence preference and sample loss are reduced; according to the invention, the immunoprecipitation process is further optimized, and the non-specific binding of the s9.6 antibody is reduced as much as possible while the yield is ensured; a single-chain library establishment scheme is designed, the chain specificity is reserved, the library establishment efficiency is