Caries activity detection reagent based on resazurin method and preparation method of caries activity detection reagent

The invention relates to the technical field of caries activity detection reagents, and provides a caries activity detection reagent based on a resazurin method and a preparation method thereof. The detection reagent provided by the invention is prepared from the following components: water, sorbito...

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Bibliographische Detailangaben
Hauptverfasser: XIE LUHAN, ZHANG JIAXIN, GU YULI, XUE YUTING
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention relates to the technical field of caries activity detection reagents, and provides a caries activity detection reagent based on a resazurin method and a preparation method thereof. The detection reagent provided by the invention is prepared from the following components: water, sorbitol, glyceraldehyde 3-phosphate, L-(cysteine), resazurin and a thickening agent. According to the invention, the resazurin is taken as an indicator, under the action of dehydrogenase, the resazurin is reduced by reducing hydrogen transmitted by the dehydrogenase, the original bluish violet is reduced to amaranth, red or white, and the risk of suffering from caries is evaluated according to the color change of the resazurin; sorbitol is used as a substrate, so that specific recognition of streptococcus mutans is realized, and the accuracy of a detection result is improved; 3-glyceraldehyde phosphate and L-cysteine are added, so that the reaction rate is increased, and the detection speed is further increased; by addin