Method for producing theabrownin by liquid fermentation of aspergillus cristatus (MK346334, NCBI)

The invention discloses a method for producing theabrownin through liquid state fermentation of aspergillus cristatus (MK346334, NCBI), and belongs to the field of deep processing of tea leaves. Comprising the following steps: 1) extracting autumn green tea in boiling water for 45 minutes, cooling, and centrifuging to obtain clear tea soup; (2) sub-packaging the tea soup into 1L conical flasks, sterilizing for 15 minutes at 121 DEG C by using a vertical steam sterilization pot, and cooling in a super clean bench to obtain sterile tea soup; (3) inoculating the aspergillus cristatus spore suspension into the sterile tea soup; 4) performing liquid fermentation to obtain fermented tea soup; and 5) filtering the fermented tea soup to obtain clarified tea soup, carrying out vacuum concentration on the tea soup, sterilizing at 80 DEG C for 30 minutes, dialyzing the sterilized solution for 2 days by using a 3500Da dialysis bag, carrying out rotary evaporation on the dialyzed solution, taking the concentrated solution