Biosynthesis method and bio-enzyme of idoxaban tosylate chiral intermediate

The invention relates to the technical field of enzyme engineering, in particular to a biosynthesis method and bio-enzyme of an esdoxaban tosylate chiral intermediate, the method is characterized in that a compound II is used as an initial raw material, in the presence of a buffer solution, a cosolvent, a coenzyme PLP and an amino donor, the compound II is catalytically converted into a compound I through the bio-enzyme, and the reaction route is # imgabs0 #, the nucleotide sequence of the biological enzyme is as shown in SEQ ID NO.3, and the amino acid sequence of the biological enzyme is as shown in SEQ ID NO.4. The method provided by the invention reduces the use of chemical catalysts in the production process of the esdoxaban tosylate intermediate, is simple, mild in reaction condition and environment-friendly, and has a wide application prospect. 本发明涉及酶工程技术领域，尤其是一种甲苯磺酸艾多沙班手性中间体的生物合成方法及生物酶，该方法由化合物II作为起始原料，在缓冲液、助溶剂、辅酶PLP、氨基供体的存在下，经生物酶催化转化为化合物I，其反应路线为，#imgabs0#其中，生物酶的核苷酸序列如SEQ ID NO.3所示，氨基酸序列如SEQ ID NO.4所示。