Bst DNA polymerase mutant with enhanced thermal stability as well as preparation method and application of Bst DNA polymerase mutant

The invention discloses a thermal stability enhanced Bst DNA polymerase mutant as well as a preparation method and application thereof, and relates to the technical field of biology. The invention specifically discloses amino acid mutation of the Bst DNA polymerase mutant on an amino acid sequence of a wild type Bst DNA polymerase large fragment, wherein the amino acid mutation comprises H31I, C91K, S258D and T307P; the amino acid sequence of the wild type Bst DNA polymerase large fragment is as shown in SEQ ID NO: 1. Compared with a wild type Bst DNA polymerase, the Bst DNA polymerase has higher thermal stability, the yield of reaction products is higher, and the Bst DNA polymerase has a good application prospect in isothermal amplification. 本发明公开了热稳定性增强的Bst DNA聚合酶突变体及其制备方法与应用，涉及生物技术领域。本发明具体公开了该Bst DNA聚合酶突变体在野生型Bst DNA聚合酶大片段的氨基酸序列上进行氨基酸突变，所述氨基酸突变包含H31I、C91K、S258D和T307P；所述野生型Bst DNA聚合酶大片段的氨基酸序列如SEQ ID NO:1所示。相比于野生型Bst DNA聚合酶，其具有更高的热稳定性，且反应产物得率更高，在等温扩增中具有很好的应用前景。