Purification method of anti-canine IL-31 monoclonal antibody
The invention discloses a purification method of an anti-canine IL-31 (interleukin-31) monoclonal antibody. The purification method comprises the following steps: A, balancing an affinity chromatography column by using an equilibrium buffer solution; b, loading the cell fermentation supernatant cont...
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| creator | QIN XIAORUI FANG CHAODONG ZHANG JIUCHUN |
| description | The invention discloses a purification method of an anti-canine IL-31 (interleukin-31) monoclonal antibody. The purification method comprises the following steps: A, balancing an affinity chromatography column by using an equilibrium buffer solution; b, loading the cell fermentation supernatant containing the anti-canine IL-31 monoclonal antibody to an affinity chromatography column; c, balancing the affinity chromatography column by using the equilibrium buffer solution again until the cell fermentation supernate completely flows through the affinity chromatography column; d, leaching the affinity chromatography column by using a leaching buffer solution; e, balancing the affinity chromatography column by using the equilibrium buffer solution again; and F, eluting the affinity chromatography column by using an eluent, and collecting the eluent containing an elution peak of the anti-canine IL-31 monoclonal antibody, so as to obtain a purified solution of the anti-canine IL-31 monoclonal antibody. The content |
| format | Patent |
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The purification method comprises the following steps: A, balancing an affinity chromatography column by using an equilibrium buffer solution; b, loading the cell fermentation supernatant containing the anti-canine IL-31 monoclonal antibody to an affinity chromatography column; c, balancing the affinity chromatography column by using the equilibrium buffer solution again until the cell fermentation supernate completely flows through the affinity chromatography column; d, leaching the affinity chromatography column by using a leaching buffer solution; e, balancing the affinity chromatography column by using the equilibrium buffer solution again; and F, eluting the affinity chromatography column by using an eluent, and collecting the eluent containing an elution peak of the anti-canine IL-31 monoclonal antibody, so as to obtain a purified solution of the anti-canine IL-31 monoclonal antibody. 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The purification method comprises the following steps: A, balancing an affinity chromatography column by using an equilibrium buffer solution; b, loading the cell fermentation supernatant containing the anti-canine IL-31 monoclonal antibody to an affinity chromatography column; c, balancing the affinity chromatography column by using the equilibrium buffer solution again until the cell fermentation supernate completely flows through the affinity chromatography column; d, leaching the affinity chromatography column by using a leaching buffer solution; e, balancing the affinity chromatography column by using the equilibrium buffer solution again; and F, eluting the affinity chromatography column by using an eluent, and collecting the eluent containing an elution peak of the anti-canine IL-31 monoclonal antibody, so as to obtain a purified solution of the anti-canine IL-31 monoclonal antibody. The content</description><subject>CHEMISTRY</subject><subject>METALLURGY</subject><subject>ORGANIC CHEMISTRY</subject><subject>PEPTIDES</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2024</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZLAJKC3KTMtMTizJzM9TyE0tychPUchPU0jMK8nUTU7My8xLVfD00TU2VMjNz8tPzsnPS8wBSyblp1TyMLCmJeYUp_JCaW4GRTfXEGcP3dSC_PjU4oLE5NS81JJ4Zz9DQwtDCxNzc0tHY2LUAACf8i87</recordid><startdate>20240614</startdate><enddate>20240614</enddate><creator>QIN XIAORUI</creator><creator>FANG CHAODONG</creator><creator>ZHANG JIUCHUN</creator><scope>EVB</scope></search><sort><creationdate>20240614</creationdate><title>Purification method of anti-canine IL-31 monoclonal antibody</title><author>QIN XIAORUI ; FANG CHAODONG ; ZHANG JIUCHUN</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN118184779A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2024</creationdate><topic>CHEMISTRY</topic><topic>METALLURGY</topic><topic>ORGANIC CHEMISTRY</topic><topic>PEPTIDES</topic><toplevel>online_resources</toplevel><creatorcontrib>QIN XIAORUI</creatorcontrib><creatorcontrib>FANG CHAODONG</creatorcontrib><creatorcontrib>ZHANG JIUCHUN</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>QIN XIAORUI</au><au>FANG CHAODONG</au><au>ZHANG JIUCHUN</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Purification method of anti-canine IL-31 monoclonal antibody</title><date>2024-06-14</date><risdate>2024</risdate><abstract>The invention discloses a purification method of an anti-canine IL-31 (interleukin-31) monoclonal antibody. The purification method comprises the following steps: A, balancing an affinity chromatography column by using an equilibrium buffer solution; b, loading the cell fermentation supernatant containing the anti-canine IL-31 monoclonal antibody to an affinity chromatography column; c, balancing the affinity chromatography column by using the equilibrium buffer solution again until the cell fermentation supernate completely flows through the affinity chromatography column; d, leaching the affinity chromatography column by using a leaching buffer solution; e, balancing the affinity chromatography column by using the equilibrium buffer solution again; and F, eluting the affinity chromatography column by using an eluent, and collecting the eluent containing an elution peak of the anti-canine IL-31 monoclonal antibody, so as to obtain a purified solution of the anti-canine IL-31 monoclonal antibody. The content</abstract><oa>free_for_read</oa></addata></record> |
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| title | Purification method of anti-canine IL-31 monoclonal antibody |
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