Method for detecting content of polylysine intermediate based on ratio-type fluorescence sensor

The invention relates to the technical field of biological analysis and detection, and provides a ratio type fluorescence sensor for detecting the content of a polylysine intermediate, the ratio type fluorescence sensor is CDs (at) SiO2 (at) FITC (at) MIPs, and comprises an internal standard core CDs (at) SiO2 and an imprinting shell FITC (at) MIPs coating the internal standard core; the invention also provides a method for detecting the content of the polylysine intermediate by using the ratio type fluorescence sensor. The ratio type fluorescence sensor CDs (at) SiO2 (at) FITC (at) MIPs is used for detecting the content of the epsilon-polylysine intermediate in the microbial fermentation liquid. According to the technical scheme, the problems of low selectivity and low accuracy when the content of the polylysine intermediate is detected in the prior art are solved. 本发明涉及生物分析检测技术领域，首先提出了检测聚赖氨酸中间体含量的比率型荧光传感器，比率型荧光传感器为CDs@SiO2@FITC@MIPs，包括内标核CDs@SiO2和包覆在内标核外的印迹壳FITC@MIPs；还提出了比率型荧光传感器检测聚赖氨酸中间体含量的方法，采用比率型荧光传感器CDs