Rapid clinical proteome sample treatment method

The invention relates to a rapid clinical proteome sample treatment method, which is a rapid treatment method integrating protein extraction, protein denaturation, reduction and protein enzymolysis, and comprises the following steps: firstly, adding a protein reducing agent into a proteome sample, a...

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Hauptverfasser: YUAN HUIMING, ZHANG LIHUA, ZHANG YUKUI, ZHAO NAN, QU BOXIN, WANG LIMING, YANG KAIGUANG
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention relates to a rapid clinical proteome sample treatment method, which is a rapid treatment method integrating protein extraction, protein denaturation, reduction and protein enzymolysis, and comprises the following steps: firstly, adding a protein reducing agent into a proteome sample, and rapidly realizing protein extraction, denaturation and reduction under a high-temperature condition. Sub-packaging in an 8-linked tube or a 96-well plate, adding protease, and carrying out enzymolysis under the irradiation of ultraviolet light with proper intensity; and after centrifuging, collecting an enzymolysis product in the supernatant, and carrying out liquid chromatography-mass spectrometry analysis. According to the method, the problems of complicated steps, low recovery rate, long sample treatment time and the like of a traditional free solution treatment method can be solved, and the efficiency and flux of clinical plasma proteome sample treatment are remarkably improved. 本发明涉及一种快速临床蛋白质组样品处理方法,是一种集蛋白质