Recombinant bacterium of active PCP protein and preparation method of PCP protein

The invention relates to a recombinant bacterium of an active PCP protein and a preparation method of the PCP protein, a target gene of a fungal immunomodulatory protein is constructed into an expression vector, then the expression vector is transformed into host bacteria to obtain the recombinant bacterium expressing the active PCP protein, a transformant with high Zeocin resistance is further obtained through screening, and finally fermentation is carried out in a bioreactor to obtain the recombinant bacterium of the active PCP protein. The obtained supernate contains a large amount of recombinant active PCP protein. Mouse experiments prove that the recombinant PCP protein has a remarkable enhancement effect on the immunocompetence of an organism, and has a good application prospect in the aspects of improving immunity, resisting tumors and the like. 本发明涉及一种活性PCP蛋白的重组菌及PCP蛋白的制备方法，将真菌免疫调节蛋白的目标基因构建至表达载体中，再转化至宿主菌中得到表达活性PCP蛋白的重组菌，进一步经筛选得到高Zeocin抗性的转化子，最后在生物反应器中进行发酵，所得上清液中含有大量的重组活性PCP蛋白。该重组PCP蛋白经小鼠实验证实对机体的免疫能力有显