Fungus Aspergillus meso, extracellular polysaccharide thereof and application of extracellular polysaccharide

The invention discloses a fungus Aspergillus sp., an exopolysaccharide thereof and an application of the exopolysaccharide, the fungus Aspergillus sp. Is inoculated in a fermentation medium for fermentation culture, and a fermentation broth is obtained; removing thalli and proteins from the fermenta...

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Bibliographische Detailangaben
Hauptverfasser:	YANG JIAJIA, ZHONG SAIYI, KUANG WEIYANG, HUANG RIMING, XIA XUEWEI, ZHANG XIAOYONG
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS BASED THEREON COMPOSITIONS THEREOF CULTURE MEDIA DERIVATIVES THEREOF ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC MACROMOLECULAR COMPOUNDS POLYSACCHARIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS THEIR PREPARATION OR CHEMICAL WORKING-UP VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a fungus Aspergillus sp., an exopolysaccharide thereof and an application of the exopolysaccharide, the fungus Aspergillus sp. Is inoculated in a fermentation medium for fermentation culture, and a fermentation broth is obtained; removing thalli and proteins from the fermentation liquor, and dialyzing to obtain an exopolysaccharide crude extract; adsorbing pigment by the crude extracellular polysaccharide extract through a macroporous resin column, and carrying out chromatographic purification on the crude extracellular polysaccharide extract through a DEAE Fast Flow anion exchange column to obtain the extracellular polysaccharide with the molecular weight of 9015Da. The structural formula of the exopolysaccharide is determined through analysis of composition of monosaccharide, methylation, infrared spectroscopic analysis and nuclear magnetic resonance spectrum analysis. On the cellular level, preliminary experiments prove that the compound has remarkable immunocompetence on RAW264.7 m