Preparation method of fibroin fiber-silk fibroin biological composite gel dura mater

The invention discloses a preparation method of a fibroin fiber/silk fibroin biological composite gel dura mater. The preparation method comprises the following steps: degumming raw silk, silk floss or cocoon shells to obtain fibroin fibers or fibrous fibroin, treating the fibroin fibers or fibrous fibroin with a NaOH solution to obtain dispersed fibroin microfibers or clustered fibroin microfiber bundles, and pressing the dispersed fibroin microfibers or clustered fibroin microfiber bundles into fibroin microfiber sheets or fibroin microfiber bundle sheets; and further laminating, infiltrating and gelatinizing by using a silk fibroin solution to prepare the silk fibroin/silk fibroin composite gel material. The composite dura mater prepared by the invention has higher mechanical properties and excellent biocompatibility, and can effectively protect and repair spinal cord operations. 本发明公开了一种丝素纤维/丝素蛋白生物复合凝胶硬脊膜的制备方法。本发明将生丝、或者丝绵、或者茧层脱胶获得的丝素纤维或纤维状丝素，再用NaOH溶液处理，获得分散状的丝素微纤维或集束状的丝素微纤维束，并压成丝素微纤维片或者丝素微纤维束片；进一步采用丝素蛋白溶液