Precipitator composition for DNA extraction and DNA extraction method using same

The invention provides a precipitant composition capable of effectively removing substances hindering microbial DNA separation and purification and a method for extracting high-concentration and high-purity DNA by using the precipitant composition, and particularly relates to an impurity precipitant...

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Bibliographische Detailangaben
Hauptverfasser: MENG-HOEGARIGER ARNODARI, AHN, JANG-HYUK, JEON SUN-MIN, KIM MIN-HEE
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The invention provides a precipitant composition capable of effectively removing substances hindering microbial DNA separation and purification and a method for extracting high-concentration and high-purity DNA by using the precipitant composition, and particularly relates to an impurity precipitant composition, a method for extracting DNA by using the precipitant composition and a method for amplifying DNA extracted by using the precipitant composition. Wherein the impurity precipitant composition is used for precipitating and removing impurities which hinder DNA separation and purification in the process of splitting cells in a sample and extracting DNA from the cells, and the impurity precipitant composition comprises 1.3 to 2.1 percent (w/v) of phosphotungstic acid hydrate, 1.2 to 1.8 percent (w/v) of zinc acetate dihydrate, 13.0 to 19.0 percent (v/v) of acetic acid and the balance of water. 本发明提供一种有效去除阻碍微生物DNA分离纯化的物质的沉淀剂组合物及使用该沉淀剂的高浓度、高纯度DNA提取方法,具体涉及一种杂质沉淀剂组合物、使用所述沉淀剂组合物的DNA提取方法及由此提取的DNA的扩增方法,其中所述杂质沉淀剂组合