Circular RNA circMYLK and application method thereof in targeted therapy of cervical cancer

The invention belongs to the technical field of biological medicines, and discloses a circular RNA circMYLK and an application method thereof in targeted therapy of cervical cancer. A preliminary experiment and bioinformatics analysis prompt that a circMYLK-miR-148a-3p-DNMT1 signal axis has a molecu...

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Bibliographische Detailangaben
Hauptverfasser:	XU QINGZHONG, JIANG XIANGJU, YANG SHUAI, WANG YIRAN, YANG QIQI, ZHANG XU, LI XIAOLIN, ZHANG LI
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY HUMAN NECESSITIES HYGIENE INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention belongs to the technical field of biological medicines, and discloses a circular RNA circMYLK and an application method thereof in targeted therapy of cervical cancer. A preliminary experiment and bioinformatics analysis prompt that a circMYLK-miR-148a-3p-DNMT1 signal axis has a molecular regulation and control relationship in CC, and the circMYLK-miR-148a-3p-DNMT1 signal axis, as a molecular hub, closely participates in a plurality of signal channels related to CC. According to the present invention, the expression difference of circMYLK, miR-148a-3p and the target gene thereof in cervical cancer is determined through qPCR, Westernblot, nucleic acid amplification and other methods, and the influence of circMYLK and miR-148a-3p on the occurrence and development mechanism of cervical cancer is further discussed on the animal and cell level through the addition of an inhibitor, the RNA interference and the overexpression of circMYLK and miR-148a-3p. And a theoretical basis is provided for further