Pelteobagrus fulvidraco SUMO removal modification system as well as construction method and application thereof

The invention discloses a pelteobagrus fulvidraco SUMO removal modification system as well as a construction method and application thereof. According to the modification system, an eukaryotic expression plasmid vector pcDNA3.1 (+) is used as a template, a fusion protein gene is inserted between two...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	GONG YULONG, LUO ZHI, TAN XIAOYING, ZHONG CHONGCHAO, DUAN FUXUAN, SONG CHANGCHUN, LIU TAO
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CARE OF BIRDS, FISHES, INSECTS CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW BREEDS OF ANIMALS PHYSICS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR SPIRITS TESTING TRAPPING VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The invention discloses a pelteobagrus fulvidraco SUMO removal modification system as well as a construction method and application thereof. According to the modification system, an eukaryotic expression plasmid vector pcDNA3.1 (+) is used as a template, a fusion protein gene is inserted between two restriction enzyme cutting sites of Hind III and Xho I, and the fusion protein gene is an SENPs coding gene carrying a tag protein gene at the 3'end. The technical limitation that the influence of single or multiple SENPs on SUMOylation modification cannot be researched is overcome, meanwhile, the blank of in-vitro construction of a pelteobagrus fulvidraco SUMOylation modification system is made up, in-vitro efficient research on the SUMOylation modification of the pelteobagrus fulvidraco is achieved, the theory of enriching the whole SUMOylation modification signal channel of the pelteobagrus fulvidraco is facilitated, and the research on the SUMOylation modification of the pelteobagrus fulvidraco is facilitated.