Method for creating watermelon male sterility germplasm through gene editing ClMMD1

The invention discloses a method for creating watermelon male sterility germplasm through gene editing ClMMD1, and belongs to the technical field of plant gene engineering. According to the method for creating the watermelon male sterility germplasm through gene editing of the ClMMD1, gene editing is conducted on the watermelon ClMMD1 through a CRISPR/Cas9 gene editing technology, the gene function is deleted, and therefore the phenotype of recessive nuclear male sterility is generated. Compared with a normal material, the watermelon male sterile line created by the method disclosed by the invention has the advantages that the fertility of male flowers is changed, and other tissues such as roots, stems, leaves, tendrils, female flowers, fruits, seeds, growth vigor and the like have no visible phenotypic change. If the created male sterile new germplasm is used for hybrid seed production, the labor cost is greatly reduced, the seed production efficiency is improved, and the important production and application