Soybean genetic transformation method

The invention discloses a soybean genetic transformation method. The method comprises the step of jointly transforming a transcription factor AtGRF5 and a spectinomycin resistance gene into soybeans. By applying the technical scheme provided by the invention, the transcription factor AtGRF5 and the...

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Bibliographische Detailangaben
Hauptverfasser: SONG SI-KYU, YU DANNA, CHEN XINGUANG, ZHANG WANGGEN, LIU QI, HAN ZHIGUO, CHENG YUNWEI
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a soybean genetic transformation method. The method comprises the step of jointly transforming a transcription factor AtGRF5 and a spectinomycin resistance gene into soybeans. By applying the technical scheme provided by the invention, the transcription factor AtGRF5 and the spectinomycin resistance gene aadA are combined to transform the soybeans, so that the transformation efficiency is greatly improved, especially the genetic transformation efficiency of main varieties of Chinese soybeans, namely the Zhonghuang 13 and the Zihuang 34 is improved; when the spectinomycin is used for screening the regenerated soybean plants, leaves of non-transgenic plants fade and turn white, so that the transgenic plants can be identified by naked eyes, the identification of the transgenic soybean plants is simple and efficient, the identification of the transgenic plants in the early stage is facilitated, the non-transgenic plants are removed, and the identification efficiency is improved. Therefore,