Application of rice E3 ubiquitin ligase BISG1 and coding gene thereof in rice disease-resistant breeding

The invention discloses application of rice E3 ubiquitin ligase BISG1 and a coding gene thereof in rice disease-resistant breeding, belongs to the technical field of genetic engineering, and discloses application of the rice E3 ubiquitin ligase BISG1 in regulation and control of rice blast resistanc...

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Bibliographische Detailangaben
Hauptverfasser: XU LI, WANG JING, SHI HUI, LU XIANG, CHEN WEIXUE
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses application of rice E3 ubiquitin ligase BISG1 and a coding gene thereof in rice disease-resistant breeding, belongs to the technical field of genetic engineering, and discloses application of the rice E3 ubiquitin ligase BISG1 in regulation and control of rice blast resistance, improvement of the rice blast resistance or cultivation of rice blast-resistant transgenic rice. An overexpression vector and a gene knockout vector of the rice BISG1 gene are constructed, the vector is transferred into a Taibei 309 receptor material, a BISG1 overexpression plant and a knockout plant are obtained, disease resistance analysis is conducted on the obtained transgenic plant, and the result shows that the knockout BISG1 gene remarkably improves the resistance level of rice to rice blast, shows that the BISG1 negatively regulates the disease resistance of the plant, and has a good application prospect. The coding gene BISG1 can be used as a target gene for improving plant disease resistance, has a goo