Physical and chemical based erythrocyte exosome extraction process and purification and loading method

The invention belongs to the field of biological medicine, and particularly relates to an erythrocyte exosome extraction process and a purification and loading method based on physics and chemistry. Specifically, the invention provides a method for efficiently preparing erythrocyte exosomes (RBCEVs) through chemical treatment (calcium ion carrier A23187) or physical treatment (micro extruder), and the exosomes obtained by the method can be loaded with drugs at a loading rate higher than that in the prior art. 本发明属于生物医药领域，具体涉及基于物理和化学的红细胞外泌体提取流程以及纯化与装载方法。具体地，本发明提供了通过化学处理(钙离子载体A23187)或物理处理(微型挤出器)高效制备红细胞外泌体(RBCEVs)的方法，所述方法所取得的外泌体可以以高于现有技术的装载率装载药物。