Medium-temperature high-activity glycogen phosphorylase and application thereof

The invention discloses medium-temperature high-activity glycogen phosphorylase and application thereof, and belongs to the field of gene engineering and enzyme engineering. According to the invention, a plasmid pET-20b (+) is used as an expression vector, Escherichia coli BL21 is used as an expression host, and the expression of the alpha-1, 4-glycogen phosphorylase derived from Thermotoga sp.SG1 in escherichia coli is realized. The invention further provides a method for producing alpha-D-grape-1-phosphoric acid by catalyzing the alpha-1, 4-glycogen phosphorylase Th alpha GP under the medium temperature condition, higher yield can be obtained, and the method can be used for industrial production in the fields of food, medicine and the like. 本发明公开了一种中温高活性糖原磷酸化酶及其应用，属于基因工程和酶工程领域。本发明以质粒pET-20b(+)为表达载体，以Escherichia coli BL21为表达宿主，实现了来源于Thermotoga sp.SG1的α-1,4-糖原磷酸化酶在大肠杆菌中的表达。本发明还提供了将α-1,4-糖原磷酸化酶ThαGP在中温条件下催化生产α-D-葡萄-1-磷酸的方法，可获得更高的产量，可用于食品、医药等领域的工业化生产。