Method for rapidly and efficiently detecting 6-thioguanine through chemical derivatization-high performance liquid chromatography

The invention discloses a method for rapidly and efficiently detecting 6-thioguanine through chemical derivatization-high performance liquid chromatography. The method comprises the following steps: S1, marking 6-thioguanine by adopting a small molecule compound to form chemical derivatization of 6-thioguanine; s2, determining 6-thioguanine by adopting a high performance liquid chromatography method; 6-thioguanine can be rapidly, accurately and sensitively marked within 3 minutes, so that the signal response of the 6-thioguanine can be improved, trace and trace 6-thioguanine in a sample or a blood sample can be separated and detected, and the separation and detection time is generally within 5 minutes. 本发明公开一种快速高效的化学衍生-高效液相色谱检测6-硫鸟嘌呤的方法，包括以下步骤：S1，采用小分子化合物标记6-硫鸟嘌呤形成6-硫鸟嘌呤的化学衍生；S2，采用高效液相色谱法测定6-硫鸟嘌呤；可在3分钟内快速、准确、灵敏地标记6-硫鸟嘌呤，从而提高其信号响应实现对样品或血液样本中6-硫鸟嘌呤微量、痕量的分离与检测，分离检测时间一般在5分钟内。