Fungus immunomodulatory protein FIP gene, protein coded by same and application

The invention relates to an artificial gene of a fungal immunomodulatory protein FIP. The nucleotide sequence of the gene of the fungal immunomodulatory protein is shown as SEQ ID No. 1. The invention also relates to a protein coded by the gene, and the amino acid sequence of the protein is shown as...

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Bibliographische Detailangaben
Hauptverfasser:	TIAN CHENG, BU XIUFEN, LI HONGBO, YUE FENFANG
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to an artificial gene of a fungal immunomodulatory protein FIP. The nucleotide sequence of the gene of the fungal immunomodulatory protein is shown as SEQ ID No. 1. The invention also relates to a protein coded by the gene, and the amino acid sequence of the protein is shown as SEQ ID NO.2. The invention designs an artificial gene sequence capable of expressing the fungal immunomodulatory protein FIP at a high level, further utilizes a pET28 vector to construct recombinant plasmids and converts the recombinant plasmids into an escherichia coli expression strain, so that soluble expression of an expression product is realized, and a large amount of recombinant fungal immunomodulatory protein FIP in a soluble form is obtained; in the expression system provided by the invention, the recombinant fungal immunomodulatory protein FIP can be folded in a proper manner and keeps natural conformation, and the prepared protein shows extremely remarkable hypoglycemic activity after being fed to mice.