Modified Pae CasDinG helicase and application thereof

The invention provides a modified Pae CasDinG helicase and application thereof, and relates to the technical field of gene sequencing. The invention firstly provides a CasDinG helicase truncation with unwinding activity, and further provides a modified PaeCasDinG helicase which is used for covalentl...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	CUI YANG, LOU JIZHONG, GU TIANYAN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The invention provides a modified Pae CasDinG helicase and application thereof, and relates to the technical field of gene sequencing. The invention firstly provides a CasDinG helicase truncation with unwinding activity, and further provides a modified PaeCasDinG helicase which is used for covalently linking a WalKerA motif and a WalkerB motif in an HD1 structural domain through a connecting molecule, and the modification improves the binding stability of the PaeCasDinG helicase and polynucleotides. 本发明提供了一种修饰的Pae CasDinG解旋酶及其应用，涉及基因测序技术领域。本发明首先提供了具有解旋活性的CasDinG解旋酶截短体，进一步提供了通过连接分子将HD1结构域中WalKerA基序和WalkerB基序共价连接的经修饰的Pae CasDinG解旋酶，所述修饰提高了本发明Pae CasDinG解旋酶与多核苷酸结合的稳定性。