Genetic engineering strain for enhancing co-production of L-isoleucine and L-valine and application of genetic engineering strain

The invention provides a gene engineering strain for enhancing co-production of L-isoleucine and L-valine and application of the gene engineering strain. Comprising the following steps: expressing an acetolactate synthase gene through an inducible promoter by the genetic engineering strain, so as to realize controllable conversion of carbon flow from pyruvic acid to acetolactic acid. By introducing the plasmid capable of inducing strong expression of acetolactate synthetase, co-production of isoleucine and L-valine is realized, the production efficiency and yield are improved, the raw material conversion rate is improved, and the method has the advantages of high production efficiency and suitability for industrial production. 本发明提供了一种强化L-异亮氨酸和L-缬氨酸联产的基因工程菌株及其应用。包括所述基因工程菌株通过诱导型启动子表达乙酰乳酸合成酶基因，从而实现碳流从丙酮酸向乙酰乳酸的可控转化。本发明通过引入可诱导强表达乙酰乳酸合成酶的质粒，实现了异亮氨酸和L-缬氨酸的联产，并且提高了生产效率和产量，提高了原料转化率，并具有生产效率高且适合于工业生产的优点。