Modified alphaviruses with heterologous non-structural proteins

The present disclosure relates to the field of molecular virology, including nucleic acid molecules comprising a modified viral genome or replicon (e.g., self-replicating RNA), pharmaceutical compositions containing the nucleic acid molecules, and the use of such nucleic acid molecules and compositions for producing a desired product in a cell culture or in vivo. Methods for eliciting an immune response in a subject in need thereof and methods for preventing and/or treating various health conditions are also provided. 本公开文本涉及分子病毒学领域，包括包含经修饰的病毒基因组或复制子(例如，自复制RNA)的核酸分子、含有所述核酸分子的药物组合物以及此类核酸分子和组合物用于在细胞培养物或活体中产生所需产物的用途。还提供了用于在有需要的受试者中引发免疫应答的方法以及用于预防和/或治疗多种健康状况的方法。